Objective:to identify the blood group of the person using forward test
Introduction :ABO grouping is performed before produce such as blood transfusion or organ transplantation,an individual should be transfused of the same blood ABO group.the rule to follow in transfusion blood is adviod giving the patient an antigen that he do not have ,the slide test detected the A or B antigens on the red blood cell using the principle of agglutination,this produce called direct or forward grouping and is accomplished by combining the patient blood cell with a known antiserum and observing for agglutination,if the antigen present on the cells corresponded to the antibody in the serum,the antibody will bind to the antigen and cause clumping of the cells or agglutination,if the antigen is not present on the cells,no agglutination will observed.
Material : tile,serum,fresh blood,venipunture,alcohol
Procedure :
1. 1 drop of blood are placed on 3 quartend tile
2. Added anti serum for each on it
3. Used the toothpick to mixed well the blood with the anti serum
4. Observe what happen
Discussion
For the forward test it use the antibody serum to identify the blood type.this because the blood type are having the antigen on the surface of it,it will determined the type of the blood group like the A.B and the O group, the serum are manufactured from the factory so the serum compability are accurate and the result are confirmed as it should be.the blood taking must be freshly acquired from the patient to keep the test can be getting a good result.this test are the famous test used in determine the blood group for anyone.the reaction of the antibody and antigen making the agglutination to happen making the blood group determination are acuiqred.
Conclusion
The blood group O are the dominace in the class
References
The clinical immunohaematology notes
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