Title:red blood cell suspension

Practical 1:

Title:red blood cell suspension

Objective: to prepare 2%,5%,10% of cell suspension

Introduction :saline solution have used in immunohaematology lab for cell washing and to prepare cell suspension.1% sodium hpochlorite areprepared to clean up places contaimened by blood,suspensions of washed blood having and unknown antigens on their surface area are required for various test in the lab,the washing of cell is necessary to remove plasma ,which may interfere with the interferences with the reaction of the cells,in addition,blood group substances in plasma may neutralize the antiserum leading to false negative result,it also necessary to use red blood cell suspension of the appropriate strength for optimum reactionwith antibody molecule,the red blood cell are suspended in isotonic solution of the sodium chlorideat ph 7.0 adjusted

Procedure :

1.      1 drop of blood were put in the centrifuge tube

2.      Added saline in it until there is 1cm left from the tube mouth

3.      Then centrifuge it at 2500-3000 rpm for about 1-2 minutes

4.      After centrifuge the supernatant are removed and blood are mixed well with another saline

5.      The step 2-3 are repeated

6.      When all the blood are done centrifuge prepare 2% suspension(1 drop blood&49 drop saline),5%(1drop blood,19 saline),10 % (1drop blood,9 drop saline)

Discussion

In this experiment, the blood were added with the saline and mix up together ,the blood cell not hemolysis with the saline water making the experiment can be make to clean the blood to be tested like making the blood grouping test,or other test.some blood in the body it contain many type of distrubace in it like medication that use ,the umbricoil cord ,and other factor.the blood in this experiment were cleaned by 3 times by the centrifuge to make the substances can be removed from the blood solution. The error from the experiment can be happen like when pouring the supernatant from the tube the solution must be removed all to ensure no other substances left out in the tube.while the tube must grip on the right position,to ensure the tube not fall out or missed out from the hand.after the centrifuge done the blood now can be used for the test to it.

Result

 

Conclusion

cells suspension 3-5% have been suspended

Question

1. What is the importance of this study?

To understand the procedure of this experiment to used in the test when there  is disturbance in the result test.to cleaned the blood solution.before test to make sure the test are accurate,

2. What is Wharton jelly ?

Gelatinous substance within the umbilical cord, largely made up of mucopolysaccharides (hyaluronic acid andchondroitin sulfate). It also contains some fibroblasts and macrophages

References

Clinical imunohaematology notes