MLT-CK: brine floatation technique

Practical2

Title :brine/floatation technique

Objective :

1. To identify the purpose of reagent used

2. To identify parasite

Method :

1. 4mL stool are mixed togheter with 8mL saline

2. The wire gauze are used to filtrate the stool solution and channel it to universal bottle

3. The brine solution are filled into the it until reached the top mark

4. Placed the slide on top of the bottle mouth leaved for 30minutes,the slide are lifted and observed

result

Discussion

Brine floatation technique are used to confirmation the parasite ,the less weight of the parasite egg or adult worm can be identify by this technique, the parasite will be float to the test tube mouth or the bottle mouth and sample can be get from there, the specimen like the stool or other type. the principle of this test are used the method of a qualitative test for the detection of nematode and cestode eggs and coccidia oocysts in the faeces. It is based on the separating of eggs from faecal material and concentrating them by means of a flotation fluid with an appropriate specific gravity. the higher of the specific gravity of the parasite the more tendency the parasite are floating on the slide that placed.

the brine solution are slightly like the salt solution that float anything that less density than it ,containing the sodium chloride that will float the parasite in the solution that made up, the brine must be using the brine bottle to get the good result, after the slide were leaved on the bottle mouth it must be placed for the 30 minutes to allowed the parasite to deposited in it.

The hookworm egg are result that can be get from this test the hookworm eggs are oval or elliptical, measuring 60 µm by 40 µm, colourless, not bile stained and with a thin transparent hyaline shell membrane. When released by the worm in the intestine, the egg contains an un segmented ovum. During its passage down the intestine, the ovum develops and thus the eggs passed in feces have a segmented ovum, usually with 4 to 8 blastomeres. The trichuris are other type of parasite that can be identify from this test,the egg of this parasite are less density than the brine solution. the egg morphology barrel or spindle in shape and 50 x 20µm in size. It is brownish and has a translucent polar plug at either ends. The content of the egg is an undeveloped cell the egg of the ascaris are heavy only the unfertilized egg are the un heavy one.the ascaris also can be identify from this test the unfertilize egg because of the egg do cannot give infection to the man and it are useless also the weight are less.

Conclusion

The floatation can be used for the specific type of the parasite because some of the parasite are heavy in density and can they need other type of the test to confirm of it present.

Question

Are the brine flotation technique are same like the formalin acetyl acetate?

There are no similarities of the floatation technique and the formalin acetyl acetate because the brine deposited on the top of the brine bottle and the formalin acetate are deposited on the bottom of the tube.